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Amaxa i-scei expression vector (pcbasce
I Scei Expression Vector (Pcbasce, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/scei+expression+vector+pcbasce/s3c+expression+plasmid/pm19680010-49-3-14
Average 90 stars, based on 1 article reviews
i-scei expression vector (pcbasce - by Bioz Stars, 2026-09
90/100 stars

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Expressing:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Plasmid Preparation:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Electroporation:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Transfection:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Incubation:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.

Irradiation:

Article Title: RAD18 and Poly(ADP-Ribose) Polymerase Independently Suppress the Access of Nonhomologous End Joining to Double-Strand Breaks and Facilitate Homologous Recombination-Mediated Repair
Article Snippet: In transient transfections, 5 106 cells were suspended in 100 l of Nucleofector solution T; mixed with each of several circular-plasmid DNAs (5 g) (chicken Ku70, chicken RAD18, and I-SceI expression vector [pCBASce]); and electroporated with an AMAXA machine (Koeln, Germany) using either the A30 or B23 program.

Article Title: An approach to estimate radioadaptation from DSB repair efficiency.
Article Snippet: We introduced the I-SceI expression vector (pCBASce) by electroporation methodology using Nucleofector Kit V (amaxa AG, Cologne, Germany) (Fig. 3).36–38) The I-SceI expression vector was introduced into about 65% of the cells at 24 hr after the transfection and the expression last for 3 days incubation.37) The relatively long expression allowed us to succeed in estimating the influence of low-dose, lowdose-rate γ-rays irradiation on DSB repair, especially the effect of post-IR-exposure, as described below.



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